Nucleator-dependent intercellular assembly of adhesive curli organelles in Escherichia coli.

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Nucleator function of CsgB for the assembly of adhesive surface organelles in Escherichia coli.

Curli are surface organelles in Escherichia coli that assemble outside the bacterium through the precipitation of secreted soluble CsgA monomers, requiring the CsgB nucleator protein. Using immunoelectron microscopy and immunoblotting assays, CsgB is shown to be located on the bacterial surface and also as a minor component of wild-type curli. CsgB lacking its 20 N-terminal residues when fused ...

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Amyloid is associated with debilitating human ailments including Alzheimer's and prion diseases. Biochemical, biophysical, and imaging analyses revealed that fibers produced by Escherichia coli called curli were amyloid. The CsgA curlin subunit, purified in the absence of the CsgB nucleator, adopted a soluble, unstructured form that upon prolonged incubation assembled into fibers that were indi...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1996

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.93.13.6562